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08 May

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Urinary Cobalt Excretion in Horses Supplemented with 1 mg Cobalt in Addition to Normal Dietary Levels

Background

Cobalt is a trace element, required by horses in tiny amounts. It is necessary for vitamin B12 synthesis, and as a result, is a key nutrient affecting several aspects of energy production, metabolism, appetite, and health.  Along with copper, iron, B-vitamins, and other cofactors, it is involved in red blood cell production.   Cobalt is naturally occurring in feeds, in quantities reflective of the soils in which they are grown.  NRC 2011 lists several regions with soils known to be deficient in cobalt, including New Zealand and Australia.

National Research Council (NRC) is an organization that compiles and publishes the internationally recognized standards for nutritional requirements for most species, in various stages of life, environmental conditions, and levels of work. NRC 2007 standards indicate that horses in hard work or under stress require at least 0.1mg of cobalt per kilogram of dry matter intake. Your average 500kg racehorse can be expected to eat 2% of their body weight per day, which would be 10kg of feed on a dry matter basis. 10kg dry matter intake X 0.1mg cobalt required per kg dry matter = 1mg of cobalt required per day for normal health. According to NRC, resting horses require about half of that.  NRC 2011 standards list reduced minimum requirements, ranging from 0.5mg to 0.6mg, depending on age and level of work.

Cobalt has become a very significant issue in racing over the past year or so. Following positive tests in Australia, racing authorities have made cautionary statements about the administration of cobalt to horses, and it has been reasonably well publicized that administering it at levels that result in the excretion of more than 100-200 micrograms of cobalt per litre of urine (depending on the racing jurisdiction) will result in a positive test. What hasn’t been explained is how much cobalt you can safely feed before those levels are reached. Racing jurisdictions have been working towards finding that threshold but have not yet released any information.

On a more basic level, horsemen and veterinarians have been provided with very limited information about the impact of “normal” levels of cobalt in the feed on the cobalt levels in urine. “Normal” levels may be significantly less than the threshold doses that will eventually be established. Instead, regulatory authorities have said that cobalt deficiencies are not common in horses, and they have recommended that it should be eliminated, as much as possible, from the equine diet until data is published indicating the maximum amount that can be fed.

In general, feed companies use NRC guidelines when developing formulations, so most complete feeds will contain at least 1mg of cobalt per day, when fed as directed. Many racing stables, however, feed simple diets, comprised of unimproved pasture, home-grown hay, haylage, and chaff, supplemented with oats or barley. It is often necessary to top-dress these rations with broad-spectrum vitamin and mineral supplements to ensure that all nutrient requirements are met.  Based on the cautionary statements issued by some racing authorities about feeding cobalt, horsemen are now unsure if it is safe to continue feeding their usual preparations. Withholding all nutritional supplements, however, may not be the best approach, as far as equine health and welfare are concerned.  Some diets will almost certainly be left deficient in at least some vitamins and minerals.

Pro-Dosa BOOST is a complete, balanced, bioavailable multi-nutrient paste, developed to replace nutrients lost in hard work or required in greater doses when horses are under stress as a result of travel, racing, competition, or illness. Nutrients are provided in doses that directly reflect NRC requirements, so Pro-Dosa BOOST contains 1mg of cobalt. It is generally only used once or twice a week, on days in which horses are under greater stress, and is not a daily supplement. It has been widely used in racehorses and competitive horses of all types in 35 countries for the past 14 years. It has undergone in vivo clearance testing numerous times across Australasia and has previously been fed extensively in those markets, right up until race day, without incident. Currently, Pro-Dosa International Ltd fields weekly enquiries about the safety of feeding Pro-Dosa BOOST and/or normal rations to racehorses.

Purpose

While the industry awaits peer-reviewed research to be published by regulatory agencies regarding maximum amounts of cobalt that can be fed on a daily or weekly basis without producing a positive test, we wanted to be able to answer questions about the cobalt levels in urine after feeding small amounts of cobalt, that are simply reflective of accepted requirements.

The purpose of our study was to measure the amount of cobalt excreted in the urine of horses in race training, fed a combination of typical, prepared feeds and home-grown forages, and then to quantify the increase in the amount of cobalt excreted in urine following the administration of an additional 1mg cobalt (provided as cobalt sulphate in Pro-Dosa BOOST) a very bioavailable form of the trace mineral.

Project and Study Design

We have conducted a fairly short, practical study of urine cobalt levels in horses given a small quantity (1mg) of cobalt sulphate, a dose that reflects the nutrient requirements of a horse in hard work as published by NRC (National Research Council) in 2007.

Specifically, the following are the steps we followed:

On day 1, we fed a group of horses a standardized diet in which cobalt content was measured, and then we collected a baseline sample of urine in which cobalt was also measured. (Sample 1)

A single syringe of Pro-Dosa BOOST, made without any cobalt, was administered to each horse (2), and urine samples were collected over 36 hours, at intervals reflecting label directions and typical race day schedules.  These were again analyzed for cobalt. This first treatment represented the negative control treatment. (Samples 3-7)

On day 3, a single syringe of Pro-Dosa BOOST containing 1mg of cobalt (as it normally does) was fed to each horse (8). Urine samples were collected at the same intervals for a further 36 hours, and these were also analyzed for cobalt. (Samples 9-13)

Hypothesis

An “hypothesis” is what is expected to occur.  Since NRC recommends the daily administration of 1mg cobalt, we expected that 1mg of cobalt would be cleared within 24 hours, and since NRC suggests that horses actually require that much cobalt, we might have expected that no waste cobalt would be excreted at all when 1mg was fed. As the standardized diet, however, was understood to contain some cobalt, we actually expected (or hypothesized) that the administration of an additional 1mg, might produce a small increase in the amount of cobalt excreted.

Results

Feed analysis was conducted at Hill Laboratories, an accredited feed testing facility.  Results indicated that the basic feed items including chaff, haylage, and barley did not, on their own, contain enough cobalt to meet requirements as set out by NRC.  Two of the prepared feeds appeared to.   Cobalt content in each feed constituent ranged from 0.009 mg/kg to 1.667 mg/kg on an as fed basis.  (See Figure 2) A complete feed analysis is available if requested.

Treatments were given and samples were collected close to the planned schedule.  Urine cobalt analyses were performed by Eurofins, Wellington, a laboratory accredited for that test.  They are not a racing laboratory, however.

While the trends appeared to be quite consistent, there was some individual variation between horses, with one horse excreting double the cobalt compared to the other two at baseline.  (See Figures 3 & 4)  The horses were all of similar size, ranging from 425-450kg, and one of the two larger horses had the higher cobalt excretion values.

Results indicated a slight reduction in urinary cobalt following the administration of the portion of Pro-Dosa BOOST containing no cobalt (the negative control), and in two of the horses, that reduction was to a level below detectable limits.  The reduction in cobalt concentration in urine occurred between 4 hours and 12 hours, with levels staying low until some time after 20 hours.  By 28 hours after feeding Pro-Dosa BOOST without cobalt, cobalt excretion had returned to levels close to baseline.

After feeding the portion of Pro-Dosa BOOST with 1mg of cobalt, urinary excretion of cobalt remained essentially unchanged from baseline.  Statistical analyses performed by the University of Auckland, Department of Statistics, demonstrated no statistical significance of the very slight increases in two horses or the slight decrease the other.

To get a perspective on the amount of cobalt excreted after feeding a basic, un-supplemented ration and the additional effect of feeding 1mg of cobalt in comparison to threshold levels that would produce a positive drug test, see Figure 5.

Figure 5:  Urinary Cobalt Excretion in this Study Compared with Industry Thresholds 

Discussion and Conclusion

It is important to understand that the number of horses used in this study was very small.  A larger number of horses and a larger number of samples would be needed to draw statistically significant conclusions.  Until this study was completed, we did not know what sort of data we might get, if any at all, and the cost was quite high. We had intended to repeat the study in a second and third stable, but only after looking at preliminary data.  Unfortunately, it was more difficult to collect the samples than expected.  After the first day or two of urinating in pots, the horses stopped cooperating, and the final sample could only be collected from one horse.  With no additional data to compare it to, that data was discarded.  Had the urine collection been easier, a second, larger group of horses could have been used to obtain more results.  As it stands, few stables would be likely to volunteer to participate in a follow up study, especially if they talked to the trainer and staff who were involved in this one.

It was originally planned that blood samples would be taken in addition to urine, but that had to be abandoned as no laboratory that was accredited to do cobalt analysis in blood or serum was willing to participate. If a suitable laboratory can be identified, serum cobalt may be measured in a second study.  That would be easier, and I’m sure racing stables would happily participate.

Despite the limitations in our data set, the results were interesting, and we have achieved some of our goals.  We are now able to answer questions regarding the amount of cobalt excreted in urine compared to threshold levels, in a small group of horses in training, fed a diet, fairly typical of racing stables in the Auckland, New Zealand region.

In general, urinary cobalt was quite low compared to threshold levels, with our group averaging a baseline excretion of only 2.97 mcg/L while the threshold for drug testing has been set 67 times higher at 200 mcg/L in New Zealand and Australia. The study group of horses was eating 4.64mg of cobalt per day; with 3mg coming from one feed source, and only very small amounts coming from the forage and plain barley.  While this cannot be considered an indication of all basic feed stuffs, it is reasonable to assume that at least some un-supplemented rations, comprised of basic feed constituents, with no pre-mixed feeds included may be quite low in dietary cobalt.

Additionally, we established that feeding 1mg extra cobalt, provided as cobalt sulphate, did not significantly increase cobalt levels in urine.  Our group demonstrated a very slight reduction over 12 hours with a return to baseline levels or just slightly above by 20-28 hours.  This change was not statistically different from baseline, though.

Finally, the negative control samples showed some interesting trends.  The reduction in cobalt clearance was small, but very consistent.  It occurred between 4 and 12 hours and lasted for 20 hours.  This reduction could have resulted from increased water consumption and increased urine output, thereby reducing the concentration of cobalt. Urine output was not measured in this study, however, and nor was water consumption.  Both would be useful and may be examined in subsequent studies.

The second possible explanation is that, by feeding Pro-Dosa BOOST, which provides a broad range of elements required for metabolism, energy, and red blood cell production, the utilization of existing dietary cobalt was improved, leaving less to be excreted as waste.  In either case, the implications for health, performance, and recovery are positive.

Summary

We are now confident that feeding Pro-Dosa BOOST according to directions will not contribute to increased levels of urinary cobalt excretion.

We are comfortable that the levels of urinary cobalt excretion found in our study are much less than that which would place horses at risk for a positive drug test.

Our negative control part of the study demonstrated that Pro-Dosa BOOST reduced cobalt concentrations in urine, and that was likely due to one of the two following effects:

  1. Pro-Dosa BOOST increased water consumption, improved hydration, and increased urine output.
  2. Pro-Dosa BOOST improved cobalt utilization from the diet, reducing the amount that was excreted, unused, as waste.

If a racing laboratory can be found who is willing to do analyses on blood or who might do urine assays at a more competitive price, and if horsemen volunteer to participate, additional studies could be completed.  These would provide more confidence in the results and give trainers reassurance that feeding rations and supplements that are adequate to meet nutritional requirements will be safe when presenting horses at the races.

Pro-Dosa Int’l would like to acknowledge Derek Balle Racing Stables, Pukekohe, New Zealand for participating in the study and for collecting all the samples. We really can’t thank you enough!

07 May

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Equine Herpes Virus

Equine Herpes Virus is the most common cause of upper respiratory infections.

Clinical signs of EHV infection include a fever, runny nose, runny eyes, cough, sores in the nose, depression, loss of appetite, and swelling in the legs.

EHV can occasionally cause a neurological disease characterised by various degrees of weakness, inability to get up or stand, and even death.

EHV is also a cause of abortion in mares.

There are several different types of EHV. Type 4 causes most of the respiratory disease; type 1 causes virtually all of the abortion and some of the respiratory disease; and many types can cause the nervous system symptoms.

Vaccinate all horses except pregnant broodmares with a modified live EHV-1+4 vaccine (Duvaxyn) every three months to maintain good immunity.

At a minimum, vaccinate every 6 months. Vaccinate pregnant mares at 5, 7, and 9 months of gestation with a killed EHV-1 vaccine (Pneumabort K +1B) to prevent abortion.

Tetanus

Tetanus causes “lock jaw” and death

Tetanus is caused by a clostridial bacteria that lives in the soil

It infects animals with deep wounds

Vaccinate in the neck muscles once a year or if animal gets a deep wound.

Do not use the vaccine that combines strangles with tetanus. You will get a much higher rate of site reaction.

Strangles

Strangles is caused by a bacteria, Streptococcus Equi

It is characterised by swollen, abscessed lymph nodes, thick green nasal discharge, difficult breathing, and even death in severe cases

“Bastard strangles” involves abscesses in the chest, abdomen, or legs. These abscesses may remain for months causing severe weight loss and ill health for extended periods of time. When they eventually rupture, the animal may die.

Vaccinate in the large muscles at the back of the back legs if possible as this reduces the possibility and severity of site reactions.

Repeat every 6 months for good protection.

As this vaccine has a higher rate of reaction, many people choose to vaccinate only when there is an outbreak of the disease in the area.

A Strangles vaccine is less likely to cause a reaction if it is given alone rather than in combination with any other disease antigen in the same vaccine.

A new intra-nasal vaccine (Pinnacle) has become available in New Zealand. This has been used for several years in North America and is effective while eliminating the site reactions that were common with the injectable versions.

07 May

Protein features frequently in discussions on feeding, nutrition and performance. Alternately feared and revered, it is part of the bigger picture of conditioning/nutritional protocols that result in specific changes in body composition and performance.


The word protein comes from the Greek word ‘protos’, meaning ‘first’, because protein is the primary basic constituent of all living cells. It makes up 3/4 of the dry weight of most living cells and is involved in the formation of hormones, enzymes, antibodies and many other functions essential to life.Protein is a collection of amino acids tied together. Once the protein is consumed and digested the amino acid chains breakdown into single amino acids. To appreciate how much the racehorse depends on receiving the correct amino acids in the diet, is to be aware of how dynamic the equine system is. Every second the bone marrow makes millions of red blood cells; every four days the blood platelets and most of the lining of the gastrointestinal tract are replaced; every 10 days, most of the white blood cells are replaced and the number of muscle cells repaired or created in horses that are training and racing is huge. In addition, necessary for muscle growth and repair, strong tendons, optimal energy metabolism, increasing bone density, joint health, hoof wall thickness and greater overall soundness is the interaction of vitamins and minerals with amino acids.The horse requires a precise number and balance of amino acids. Essential amino acids cannot be synthesized by the horses system and are so-called, because it is essential that they receive them every day in the feed. The exclusion or a deficiency of even one essential amino acid from the diet will reduce total body protein synthesis. In horses where everyday maintenance is necessary and improvement with training is required, the creation of new cells is a priority. If just one amino acid in the profile is missing the creation of new cells stops.

Horses receive amino acids from the protein in both concentrates and hay. Every protein source has a different combination of amino acids. Some combinations are very good and some not so good. For this reason, the total protein of horse feed is a worthless term, unless you know the amino acid profile. For example, the feed tag might say 14% crude protein, but to the horse, if all the amino acids are not supplied, it may be only 8 or 9% usable protein. The more unusable protein the horse consumes the more he has to excrete. The feeding of unusable protein stresses the horse’s system. Excess protein can contribute to higher heart and respiratory rates, increased sweating and dehydration.

While some studies have concluded that the racing standardbred does not need additional protein, they do not take into account the quality of the protein. Poor quality protein in terms of amino acid profile, can contribute to the gradual weakening of supportive tissues, bone loss, muscle atrophy and the weakening of blood vessel walls that eventually lead to bleeding or breakdown. What is important is that the horse receives the amino acids that it needs in the correct amounts, readily digested and absorbed.

Over the centuries, horsemen and women have ground, cracked, crushed, boiled, dry-extruded and pelleted feeds for horses. They have done this to increase the digestibility of the feed and to decrease the risk of grain overload, a major player in colic, diarrhoea and laminitis. However, all amino acids have a different melting point and many of these older methods of grain processing result in damage to the amino acids and little improvement in digestibility. Soybeans and processed grain by products must go through some sort of heat process. This heat can destroy the most important essential amino acids. Feeds such as brewers grain are almost always heated at a very high temperature. This heat process destroys many valuable amino acids and makes the feed unbalanced, preventing the horse from getting the full value of the feed protein and taxing the liver and kidneys in excreting all the out of balance and, therefore, unusable amino acids.

The most recent advance in preparing feeds for horses is steam-extrusion. Whereas essential amino acid losses of up to 50% have been measured during dry-extrusion, losses during steam-extrusion are less than 5% and digestibility of the feed increases to over 90%. The application of steam-extrusion and the provision of high levels of essential amino acids in the correct balance are fundamental. Protein in general should never be fed in excess to any horse and neither should there be an overload of protein types that are poorly digested or that the horse cannot use. Essential amino acid deficiencies can occur even if diets containing excess protein and deficiencies cannot be corrected by feeding more of it, if it is not correctly profiled.

The second, separate issue is restoring protein balance after hard work or racing and encouraging the building of lean muscle mass. During hard training and racing, standardbreds damage muscle tissue as a result of the production of high levels of lactic acid and exertion. Muscle must be repaired rapidly to maintain improvement, prepare for the next training session and minimise delayed muscle soreness and stiffness. While in training or before and after racing, dietary programs must be chosen with care to facilitate quick recovery. Hard work causes a decrease in protein production that continues for a few hours after exercise has ceased. Protein in muscle is extensively broken down during exercise and this process serves a number of functions, but it is during recovery that the protein will be working hardest, repairing and building the rips and tears that occur during training.

No matter how balanced the diet is beforehand, exercise naturally causes a disruption. During exercise muscles use fuel at a very high rate and this is a catabolic process, i.e. it involves the breakdown and utilization of body reserves.  However, we are able to switch the catabolic environment into an anabolic (ie, a building of body tissues and reserves) environment after work by supplying the proper tools. Feeds chosen must contain protein that is profiled properly for amino acids, along with minerals, vitamins, fat and carbohydrates.

Nutrition is a powerful tool when used properly.  To the extent that we focus on the challenging bigger picture of conditioning/nutritional support protocol, the horse can harmonise the multiple systems necessary to reach genetic potential. High quality protein in terms of amino acid profile and availability is essential for maximum performance of the Standardbred racehorse.

07 May

Peak performance depends on the supply of energy to drive and fuel the working muscles. Providing almost three times as much energy as oats on a weight basis, oil offers many advantages in terms of energy efficiency. For both digestive and metabolic efficiency, oil is superior to grains and protein. In fact, the efficiency of ATP synthesis (i.e. the currency of energy), is around 39% for oil and 20% for carbohydrates.

In addition, calmness, as measured by spontaneous activity and reactivity (spook tests), is lower when diets are fortified with oil. Oil-enriched diets reduce the amount of metabolic heat generation, both at rest and during exercise and for the racing standardbred, this reduction in heat load can provide a competitive edge. The lower heat load lessens the need for sweating, reducing fluid loss during exercise. Studies have traditionally shown that oil-enriched diets affect working muscles by increasing oxygen uptake, increasing fatty acid utilization, sparing muscle glycogen during low intensity exercise and increasing glucose availability during intense sprinting exercise. Fat supplementation also reduces heat production, improves hydration and perhaps most importantly, improves the power : weight ratio.

Overdoing oil can result in decreased glycogen stores in the muscle, meaning the horse could “hit the wall” sooner, or have nothing left at the end. However, this does not happen until oil comprises more than 8-10% of the total diet – including hay, chaff and concentrate. So, for a 450kg horse eating 10kg of feed, oil intake would have to exceed 800-1000ml a day before there was any interference with muscle glycogen.

However, not all oils are created or utilized equally. Cold pressed oils are far healthier than solvent-extracted oils. Cold-pressed canola contains vitamin E, Coenzyme Q10, lipoic acid, omega-3 essential fatty acids and other very potent natural antioxidants. Without the addition of EPA and DHA, up to 10 times more oil is required to achieve the same levels of omega 3 activity, so for both ourselves and for hard-working horses, it is important to check that EPA and DHA are present in the oil. The maximum benefits from oil occur after two to three months, so it is best to introduce oil-enriched feeds and Omega 3 supplementation early in the program. This will allow sufficient time for metabolic adaptation to occur and ensure that the benefits of fat supplementation are realized when they are needed most.

The ratio of muscle to body fat affects the power to weight ratio – so when we want topline in a racing standardbred, we must use the combination of work and diet that will promote muscle building and not fat deposition. Just as occurs in humans, the finer details of the diet affect body composition. In addition to the type and intensity of exercise, the amount of muscle development is determined by the amino acid composition of the feed protein. Soybean meal, lupins and lucerne are well known as good sources of protein and this is because they are high in lysine.

Lysine and methionine are just two of the essential amino acids that make up protein. Just as branched chain amino acids have been found to be important in horses, on-going equine nutrition research has shown that other amino acids, including threonine are important for muscle building. Regardless of the percentage of protein in the feed, if there is not enough of each amino acid a limit will be put on muscle development and the horse will lay down cover (fat) instead of muscle.

But even if the feed contains good levels of lysine and other essential amino acids, for several reasons, they may not be available to the horse. Some feed processing techniques, such as dry-extrusion, rely on high temperatures and shearing forces which can damage lysine and other amino acids. Steam-extrusion includes moisture in the cooking process and losses are negligible and digestibility increases to over 90%.

As well as a sound daily nutrition program, strategic timing of meals pre- and post work can impact profoundly on the development of muscle power. Muscles consume vast amounts of anti-oxidants and essential amino acids during work and at the microscopic level, small strains, rips and tears occur. Correct composition and timing of feeding can take advantage of the window of opportunity created by the raised hormone levels and increased blood supply that accompany exercise. To be effective and hasten muscle recovery, the concentrate must provide anti-oxidants, amino acids and be consumed no more than two hours before or one hour after work.

The effectiveness of the diet is measured in terms of metabolic efficiency, i.e. the maximum output with the minimum production of undesirable products such as manure, acid and heat. To increase power for work, diets need to be designed to influence muscle fuel levels. But the feed chosen also influences the power : weight ratio (ratio of muscle to fat), thermoregulation (heat production and hydration) and mental attitude – all of which impact on performance and fatigue.

07 May

It has been reported that about 90% of poor performance cases can be attributed to lameness, either clinical (obvious lameness) or sub-clinical (lameness not readily visible under normal exam conditions).

It is logical that noticeable lameness causes horses to perform below their potential, but sub-clinical lameness can be an even greater problem.  Clinical lameness can be quickly recognised, investigated, and corrected.  In horses with sub-clinical lameness, however, the disease process remains undetected and untreated.  It is allowed to progress, resulting in irreversible damage to the structure of joints, secondary lameness, muscle pain, behaviour problems, impaired performance and economic losses.

Early diagnosis and intervention can stop minor problems from deteriorating, preserving long term soundness and maximising performance.

Most of my clients present every horse in their stable, on a regular basis, for physical exams.  This enables the identification of subtle or sub-clinical problems.

Clinical Lameness

A horse is clinically lame if it has a visible limp or asymmetric gait.  It will try to lift its weight off the sore leg and place more weight on the sound legs.  A “head-nod” results.  (When the sore front leg hits the ground, the horse lifts its head up to shift weight to the back legs and off the sore front leg.  When the sound front leg hits the ground, the head nods down, loading that leg excessively.)  Sometimes, when a horse is very lame in a hind leg, the horse will nod its head down to shift weight onto the front legs and off of the hind legs. Sometimes, a horse with a sore hind leg will lift its pelvis higher on the lame side (called a hip-hike).

Lameness is only visible (clinical) when one leg is relatively more painful than the opposite leg.  Both legs can be sore, but as long as the pain is unequal, the horse will protect the more sore side and the head nod will be evident.  There are various degrees of clinical lameness ranging from an inconsistent or almost imperceptible limp to an inability to bear any weight at all on the affected leg.

Sub-Clinical Lameness

Sub-clinical lameness is lameness that you can not see under normal conditions.  Bilateral lameness, lameness in all four legs, and lameness that only manifests under extreme stress or speed is sub-clinical.

Bilateral lameness is often unapparent.  If a horse’s legs are equally sore, he will not favour one and will not limp.  Instead, he will shorten his stride, develop back or muscle pain, perform and below expectations. This could include the following:- “stopping” in the last part of a race, refusing jumps, making mistakes of stride in dressage tests, tie up, blowing after working, having a longer than normal recovery, or developing behaviour problems such as pulling, bucking, and rearing. Many horses just develop a poor attitude to work. “Bleeding” or Exercise Induced Pulmonary Haemorrhage and dorsal displacement of the soft palate (“flipping the palate”) are common presenting complaints.

Some lameness only shows up at high speed or under extreme stress such as in the last part of a race.  Some will manifest only with a rider or doing particular movements like flying changes or lateral work.  Some will appear on a lunge line or on particularly hard, soft, or irregular or unstable footing.  Some only present in the cart and not in-hand. Once again, these lameness cases are often presented for performance and behaviour problems, back, or other muscle pain.

Lameness in My Practice

In my practice, the majority of horses presented for lameness or performance problems have one or more of the following:

  1. Foot pain including sole bruises, abscesses and, corns
  2. Arthritis (joint inflammation)
  3. Tendonitis (a bowed tendon) or
  4.  Suspensory Desmitis.

Bowed tendons and suspensory desmitis present as clinical lameness and there is obvious pain, heat, and swelling.

By far, the most common sub-clinical lameness or performance problems involve joint and foot pain.  In many cases these conditions are both present.

Arthritis

Arthritis is a term that means “joint inflammation” (arth-joint, itis – inflammation).   Inflammation occurs in joints when they are placed under stress in excess of what they have adapted for.  This stress can be sudden and severe (stepping in a hole, taking a bad step on poor footing, or some other accident), or it can be repetitive and low grade (wear and tear).

Horses are designed for eating grass and running away from the occasional predator.  They are designed to land flat on their feet, load bones and joints evenly from side to side, and break over the middle of their toes. Unfortunately, not many horses have perfect conformation, perfect hoof balance, or work on perfect footing so stress is not distributed evenly.  They are not born readily adapted for repeatedly pulling a sulky or carrying a rider around a track at top speed or over jumps.  The idea behind training is to gradually increase the stress on a horse causing them to adapt to the work we expect them to do.  In short, training a young horse or training a more mature horse down to race after a spell is constantly placing their joints under stress they have not adapted to. Therefore, inflammation occurs on an on-going basis in most horses in training.

Joints are made up of the ends of two or more bones which are covered with cartilage and joined together by the joint capsule.  The joint capsule is lined by the synovial membrane.  This membrane is very important as it produces the synovial fluid (joint fluid) that lubricates, protects and nourishes the joint cartilage.  In a healthy joint, synovial fluid is thick like syrup.  It is replaced every 24 hours or so on an on-going basis.

Inflammation in joints begins with synovitis and capsulitis. In synovitis and capsulitis, enzymes are produced that breakdown joint fluid, making it thin and watery.  It no longer lubricates and protects the joint properly.  With a lack of nourishment and lubrication, the cartilage surface of the joint becomes abraded.  Over a more extended period of time, the sub-chondral bone (bone underneath the cartilage in the joint) begins to change.

Over time, then, synovitis and capsulitis will progress to sub-chondral bone disease and osteoarthritis.  This entire process is referred to as arthritis or degenerative joint disease (DJD).  X-rays only show bone, so relatively advanced DJD is the first stage that is reliably visible on radiographs.

It is much better to identify and treat joint problems before they are visible on radiograph. If inflammation is stopped, the synovial membrane will make new fluid that will remain thick and sticky.  If the breakdown of synovial fluid is the only damage that has occurred, a completely normal joint environment will be restored.  If the cartilage surface has been damaged, some treatments can provide repair, and a normal joint can be created.  Once bone has changed, however, it cannot be reversed.  Thick, healthy joint fluid will stop rough bones from rubbing together in the joint, and DJD will be arrested, but a truly normal joint cannot be restored.

Treatment

Since the primary goal of therapy is to stop inflammation and to stop the progression of degenerative joint disease, the treatment of choice in most cases is intra-articular cortisone.  Cortisones are very effective anti-inflammatories, and remain the treatment of choice in human medicine for intra-articular therapy.

There are several different types of cortisone that can be used in joints.  Controlled studies have shown that all cortisones reduce inflammation and that most improve the health of joint cartilage. Triamcinolone (Vetalog, Kenalog, or Kenacort), Isoflupredone (Predef 2X), and Betamethasone (Celestone Soluspan) have all been shown to be safe or beneficial for joint cartilage. Methyl Prednisolone (Depo-Medrol or Vetacortyl) is likely safe in low doses, but can impair the healing of joint cartilage if given too frequently or in large amounts. Triamcinolone has been anecdotally linked to laminitis, but the relationship has not been confirmed.  It has never been caused in healthy horses at normal doses and has not been caused experimentally using doses up to six times those commonly used.

NSAIDS like Phenylbutazone (Bute) and Banamine tend to kill pain better than they reduce inflammation in joints, so they are not a sufficient treatment in most cases.

Once inflammation is resolved and DJD is arrested, the second goal of therapy is to restore a normal joint environment.  Hyaluronic acid (HA) is a building block for thick joint fluid, so supplementation may be useful.  HA can be administered directly into a joint, however, it does not work very well if there is a great deal of inflammation present and it is generally used in joints along with cortisone.    Studies have shown that IV HA (Hyonate or Legend) is as useful as intra-articular treatments, and recent research indicates that oral administration of HA may be helpful.

If cartilage damage has already occurred, then it can be beneficial to treat horses with a product that can stimulate joint repair or provide the building blocks for cartilage repair.  Adequan, Glucosamine Sulphate, and Pentosan may be used for this purpose.  Glucosamine Sulphate supplementation increases the body’s production of hyaluronic acid as well.

Additional medications are available to treat arthritis including some homeopathic treatments that reduce inflammation and stimulate joint healing.  The homeopathic medications I mainly use are Traumeel and Zeel.

Finally, the third goal of therapy is to prevent reoccurrence of lameness.  Adequan, Glucosamine, or Pentosan can be given regularly to reduce inflammation and repair cartilage.  They can keep inflammation at bay in sound horses in training, and they can increase the interval between joint injections in horses with lameness problems.  Optimal shoeing and good footing are of utmost importance, and adjustments to the training regimen may be helpful in some cases.

Summary

  1. The products of inflammation are enzymes that damage the joint.
  2. Early diagnosis and treatment will preserve normal joint structure and function maximizing long term soundness and performance.
  3. The first goal of therapy is to stop inflammation and, therefore, to stop the progression of degenerative joint disease.
  4. The second goal of therapy is to restore the most normal joint environment possible.
  5. The third goal of therapy is to prevent re-occurrence of the problem.

Dr.Corinne Hills

07 May

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Tying up (Exertional Rhabdomyolysis) is a common cause of poor performance in racehorses and performance horses of all types.  It is a syndrome with a variety of causes, but always involves the breakdown of muscle cells which in turn causes a serious, easily recognisable clinical condition involving muscle stiffness, pain and a reluctance to move.  Sub-clinically, it can cause poor race performance or may present as a lameness problem.

I did up a basic outline of the condition and its treatments about 15 years ago for one particular client and one particular horse.  Since then, I have sent the information out to countless others, and they have in turn passed it on.  I have been told by many people who have read this that it was helpful to them, so here it is. Please keep in mind that this is my own interpretation of the papers I have read, cases I have seen, and thoughts on the subject from other veterinarians and horsemen.  Your own veterinarian may or may not agree.  Some of the information is well documented and widely published.  Some is not.  I have not referenced any of this as I did not originally prepare the paper for general review, but only for individual clients with specific horses.  I hope it gives you something to think about, but please consult with your veterinarian regarding your own particular situation.

Clinical Signs of Tying Up

  • Shaking, sweating, stiffness, reluctance to move
  • Can look like a colic or laminitis
  • Poor performance in races or competition
  • Dark urine

Diagnosis of Tying Up

Clinical Signs
As Above

Blood Test
The muscle enzymes, Creatine Kinase (CK) and Aspartate Transaminase (AST) (and LDH in some places) are checked in serum samples (red topped blood collection tube).
CK is an enzyme that goes up quickly and down quickly (It starts to go up in a couple of hours and peaks in about 24 hours).  AST is an enzyme that goes up slowly and down slowly (It goes up in a day and back down in a week).
If CK is elevated and AST is normal, the muscle cell damage has occurred in the last few hours.  The AST has just not had a chance to rise yet.  If the AST is high and the CK is normal, that indicates that a tie-up is resolving.  The CK has gone back down to normal, but the AST has not yet had enough time to recover.  If a tie-up is on-going, both the CK and AST would remain elevated

Causes of Tying Up (or predisposing factors)

A)   Lactic Acid Damage – lactic acid is a product of the metabolism that occurs in muscle cells when they are reaching the end of a race, or when a lot of energy is stored in muscle cells and then burned off quickly

  • Too much feed or soluble carbohydrate in the feed
  • Not enough work
  • Lameness – lame horses move differently to protect sore areas.  This makes certain muscles work much harder as well as inefficiently.  This results in greater lactic acid accumulation and muscle cell damage

B)  Calcium Insufficiency – calcium is important for the normal contraction of muscle cells.  In some horses, that mechanism requires more calcium than average (apparently this is more common in exceptionally good horses)

C)  Hypothyroidism – the thyroid gland doesn’t work as well as it should, and the horse is deficient in thyroid hormone.  A blood test can identify hypothyroidism

D)  Exposure to a Virus – recently, I have noticed in the literature references to tying-up in relation to exposure to Equine Herpes Virus (Rhinopneumonitis).  I don’t know the mechanism behind this, but will update this at some point when I do.

E)  Muscle cells not properly Hydrated (not bathed in enough fluid)

  • Dehydrated (HCT or PCV above about 0.45 or 45%)
  • Not enough electrolytes or an imbalance in electrolytes or minerals
  • Fillies and mares lose more electrolytes in their urine (especially when they are in heat) compared with geldings and stallions.

Treatment of Tying Up:

A)  Reduce Muscle Enzymes in Blood

  1. Administer additional electrolytes (1 cup) and salt (handful) with water (2-3 litres) via stomach tube.  This will cause the horse to drink more water and produce more urine.  This will help to clear the high levels of muscle enzymes while protecting the kidneys (which can be damaged by myoglobin which is released when muscle cells are damaged)
  2. Anti-inflammatory
  • Tube with Dimethyl sulfoxide (DMSO) – 1 cup or 250 ml this has a 7 day withdrawal, ½ cup or 125 ml has a 4 day withdrawal (you can put 110mL in an IV electrolyte jug) this is a great anti-inflammatory and will help get the muscle enzymes back to normal
  • Give Phenylbutazone (Bute) for the first day or so (7 day withdrawal for iv)
  • Give Dexamethasone – 50 mg IV.  This is a steroidal anti-inflammatory where as Phenylbutazone (Bute) is a non-steroidal.  This means that they work by different mechanisms, so their effects will be additive.  Also, Dexamethasone may help decrease the GGT.  If GGT is much over 20-30, horses will not race well.  This is really an indicator of stress, and I don’t really know the mechanism by which it affects racing performance, but it certainly can indicate poor performance.    For some reason, the metabolites of DMSO (the things it is broken down to by the body) can result in a higher GGT.  This does not appear to be related to impaired race performance.  I don’t have a good scientific understanding of this.  Maybe I will add a discussion of GGT to this someday…

Prevention of Tying Up:

  1. Anatest – 5cc IM every 2weeks – this messes up the hormonal cycle in fillies, so they shouldn’t lose so many electrolytes in their urine.  I don’t have any idea why progesterone (in Regumate) doesn’t work as well for this, but it doesn’t seem to.  In New Zealand, you cannot use Anatest, so you must make do with progesterone.  Hydroxy-P 500 is no longer allowed in NZ either.
  2. Electrolytes – increase daily electrolyte supplementation in feed – try doubling recommended levels.  This will ensure that the horse has sufficient electrolytes available.  They will urinate out the excess anyway, but will keep what they need.  Also, a small handful of regular table salt each day will increase water consumption.  Dunstan “all-you-need” contains about 3-10 times the electrolytes contained in most feeds.  Switching your concentrated feed to Dunstan “all-you-need” will provide all the electrolytes you require.
  3. Chromium – chromium has many effects, but for racehorses, its best effects are on muscle cells.  Chromium ensures that muscle cells are bathed in more fluid and electrolytes.  It protects muscle cells from lactic acid accumulation and the damage that results from it.  (i.e. finish races better with decreased lactic acid at the end, and prevents tying up)  It also acts as a natural anabolic, causing the horse to put on more muscle than fat.  It makes insulin work better, so anything insulin is involved in will be affected by chromium (I.e. it helps boost the immune system).  Only certain forms of chromium are useful, and the one from Nutritech (Altech), Biochrome, is the best.  It is in a form that can be absorbed and used by cells well.  It is also very safe.
    Read the label on the tub.  You will need to use four times the label dose for the first two weeks, then you can cut it back to about two to three times.  That means you could feed 8 scoops (20g) every day for the next two weeks.  Then, feed 4-6 scoops daily after that.  Dunstan “all-you-need” contains Chromium.
  4. Vitamin E/ Selenium – generally, selenium is included in supplements as sodium selenite.  This is an inorganic form, and some horses are not able to absorb it or use it that well.  If you are continuing to have problems, you can feed a yeast-based selenium.  It is in an organic form that cells can easily absorb and use.  It is put out by Nutritech (Altech) as well.  Dunstan “all-you-need” contains yeast based selenium from Altech.
  5. Keep your horse sound – have a lameness exam done, treat the significant problems, and treat with glucosamine regularly to repair any damage present in joints and to prevent the reoccurrence of problems.
  6. Calcium Supplementation – feed skim milk powder regularly.  It is in a good form for absorption.  The balance between calcium, magnesium, and phosphorus is important too.
  7. Baking Soda – you can feed baking soda so long as you stop a good 24 hours before racing.  Check with your veterinarian about the dose and when to stop.
  8. Traumeel – may be useful about an hour or two before training.  It is a good muscle anti-inflammatory as is herbal so it has no withdrawal time (but you are not supposed to give it on raceday, anyway).  Give it orally.
  9. Feed High Fat, Low Soluble Carbohydrate Diet – This is the most important way to prevent tying up.  The way you feed your horse is the most vital factor.  A really good study was done on quarter horses and thoroughbreds several years ago.  It showed that if you fed horses on a high calorie diet they were more likely to tie up than if you fed them a low calorie diet.  Obviously!  It also showed that WHAT you fed affected the incidence of tie-up.  If you fed a high calorie diet that was low in soluble carbohydrate and high in fat, you had a low incidence of tying up, while a high calorie diet that was high in soluble carbohydrates caused a lot of tying up.  Soluble carbohydrates are grains like oats and barley.  Insoluble carbohydrates are hays, grass etc.  In New Zealand, a great feed to try is Dunstan “all-you-need”.  In North America, a good feed to try is Purina’s Competitive Edge.  Both are high fat, low soluble carbohydrate, fully extruded feeds.  Feed them with free choice, quality hay or grass.  Dunstan “all-you-need” has the added benefits of MSM.  This is a derivative of DMSO, a great anti-oxidant and useful in the management of lameness, as well.
  10. Don’t feed them if they are not working – if you are giving your horse a day off, cut out the grain part of the ration.  Contrary to popular belief, they will not starve to death if they do not get grain on Sunday if they are not working.  They will survive quite nicely on hay or grass.  If you are feeding Dunstan “all-you-need”, you can feed them as normally as it is low enough in soluble carbohydrate.
  11. Relax – Since nervous horses are prone to tying-up, it can be useful to treat with various things that relax the horse.  Ensure they are receiving adequate B vitamins—especially B1 (Thiamine).  Some amino acids are helpful with this as well.  Treatments like Modecate can be helpful (it is a human anti-psychotic drug that is in the same class as Acepromazine but acts for 3 months at a time).  As the FEI withholding time has increased to 90 days, Modecate is less useful.  In some racing jurisdictions, the withholding time is as little as 7 days, though, so check your local restrictions.

Dr. Corinne Hills, DVM
Equine Veterinarian & Nutritional Consultant

07 May

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This article was first posted just prior to the Horse of the Year show, a championship show that takes place in February (the peak of summer in NZ) for a wide range of equestrian sports.  While it was written for that group, the concepts apply to any horse put in a truck, float, boat, or plane.  If you are hoping to do something athletic with your horse when it arrives at its destination, this is for you.

While many people think a change is as good as a rest, horses, by nature, are homebodies.  They like their own quiet surroundings, their own friends, and routine.

Researchers at the veterinary college at University of  California, Davis (UC, Davis) have looked at several different classes of stress that affect horses in transport (you can read their research here), and another group from the University of Poland have measured oxidative stress which is a measure of inflammation that results from travel (read their research here).  You can read their papers in their entirety for yourself, but I’m basically going to summarize some of their findings, as I think they will be important for everyone travelling to Horse of the Year this week.

Several different types and sources of stress appear to affect horses when they are travelling.

  • They are removed from their homes, their routine, and their herd.
  • Water tastes different, and they may be fed from different types of feeders, on a different time schedule.
  • They will be in a confined space (truck, trailer, boat, or airplane), and they won’t be able to graze, at least while they are in transit.  In fact, they may or may not get to eat at all, and water may only be offered occasionally.  Certainly, feed and water consumption routines will be different, at best.
  • They might be stuck in a public transport next to a horse they don’t know or in your own float next to one that they don’t really get along with that well.
  • They might have to have their heads tied to prevent them biting their “friends”, and restricted head movement is another contributor to stress.
  • For some older, arthritic horses, the overall lack of free movement can be painful and emotionally stressful too.
  • While too little movement isn’t great, the flip side of the coin is that horses will have to constantly tense muscles to brace themselves to stay balanced and not fall over while you drive along New Zealand’s beautiful but winding roads.  There will be almost constant noise and vibration, and temperatures and humidity will fluctuate.
  • We had had a fabulous summer this year, and I’m certainly not complaining, but it could be pretty hot and humid for hauling your horse this weekend.  The air coming through the truck/float may not be very fresh as it may contain vehicle exhaust, dust, or ammonia from urine in the stall.

Upon arrival at your HOY, there will be a degree of organized (hopefully) chaos.  There will be trucks, people, and horses everywhere.  While you may think it is a very social, interesting, and terribly fun situation, your horse will find all the strangeness, strangers, and general mayhem to be less then comfortable.  They may not eat, drink, or rest normally upon arrival, and they may be more likely to colic.  It may take a few days of rest to recover from the trip down, but they will have to work and compete almost as soon as they arrive in many cases.  Once competition starts, they will be asked to do their very best several times in the week.  For horses, travel to HOY may result in emotional stress and physical stress.

How Did Scientists Prove That Travel Is Stressful?

Physical markers of stress were measured in horses transported over a few hours, and these included the following:

  1. CK and AST elevations confirm muscle cell damage.
    CK and AST are enzymes that are released from muscle cells when they are damaged, often by lactic acid. They are the same enzymes your veterinarian will check to confirm if your horse has tied-up.  CK goes up within 2-8 hours of the episode of muscle cell damage, and it goes back down quite quickly too.  AST takes longer to go up, needing about 12 hours.  In tying-up, CK and AST might be over 1000 or even 2000, while normal values are around 250.  In these studies, CK reached levels of about 800.  Elevations in these enzymes were moderate, and proved significant muscle cell damage.
  2. MDA, ROS (reactive oxygen species), and fibrinogen elevations confirm cell damage from oxidation and the inflammation that results.
    These are molecules that are regularly measured to demonstrate the inflammatory response to oxidative stress or the oxidative damage itself.  That sounds more complicated than it is.
    Oxidative stress is the same thing as oxidative muscle cell damage.  It occurs when exercising muscle cells produce little molecules called free radicals (ROS).  Those little molecules cause inflammation and damage to the muscles that produced them, and that process is called oxidation, oxidative stress, or oxidative muscle cell damage. The Polish team found that MDA and fibrinogen were significantly elevated, and these are indicators of inflammation and oxidative stress.
  3. Body temperature and White Blood Cell (WBC) changes demonstrate immune compromise.
    Elevated body temperatures and low white blood cell counts were common features, and both of these are related to the suppression of the immune system at times of stress.
  4. Dehydration and body weight losses confirm reduced feed consumption and either reduced water consumption or increased fluid losses.
    Dehydration, as you might expect, was a feature, especially where horses were transported in warmer weather.  It was thought to be related to the disruption in normal feed and water consumption patterns along with their response to heat.  On average, the UC Davis group reported that horses lost 6% of their body weight due to reduced feed and water consumption in addition to dehydration.
  5. Variations in heart rate and cortisol increases indicate hormonal and biological responses to emotional stress.
    Cortisol, a hormone directly elevated at times of stress was found to be significantly increased as was the incidence of heart rate variability.  These findings might tell you that your horse will actually feel stressed.

What Does All That Mean for You and Your Horse?

So as I said, researchers proved that muscle cells are damaged, inflammation develops, horses get dehydrated, and lose weight.  It can mean more than that, though, as these changes make horses more susceptible to disease conditions.  It is well known, and studies have confirmed, (several studies are referenced in the Polish group’s paper that you can look up and read if you wish) that these changes can lead to tying-up, pneumonia, and colic, to name only a few.  If you are in any doubt that these conditions may be developing, call a veterinarian immediately.

Wow! This great trip for you may not be so great for your four-legged friends.  What can you do to make it better for them?  The researchers at UC Davis had several suggestions.  In addition to the practical and obvious things like…

  • make sure your truck is in good repair and clean,
  • make sure your horses are well hydrated before they travel and have eaten well,
  • make sure they eat and drink well while away,
  • plan your route and limit the time spent travelling each day.

They came up with a few that were a bit more interesting…

  • Horses that were allowed to face backward when travelling appeared to have lower markers for stress and were less likely to suffer illness, as air quality and their ability to clear their airways was improved.   As always, there are exceptions.  A small group had greatly increased stress when facing backward and really wanted to travel facing the front.
  • In all cases, the maximum possible head freedom was good (so don’t tie your horses short unless you must), and
  • most of all, horses should be given three full days of rest to allow for complete recovery from the ordeal.

Three days of rest is probably not very realistic for most people.  Your classes may start the day you arrive or the day after.  Preventing as much of the physical results of stress as possible will be important if there isn’t time to allow for full recovery from travel, so if you can’t practically follow these recommendations, read our “Letter to Equestrian Sport Competitors” found on the NZ page of the Where to Buy section to get some tips for how to use nutrition to help your horse cope with the stresses of and recover from travel.

07 May

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I thought I was finished.  I celebrated being finished, in fact… and then I thought about product quality management and security.

How do you know if the product you are looking at contains what is says it does; only a fraction of what it says it has; or way more than it is supposed to have?  And even more alarmingly, how do you know it doesn’t contain contaminants that aren’t supposed to be there?

There was an interesting study presented at the AAEP (American Associate of Equine Practitioners) several years ago in which several nutraceuticals were analysed and their actual contents were compared with label claims.  Those products were found to contain anywhere between 10 and 200% of the active ingredients that they were supposed to have.  Apparently, this is a more wide-spread problem then you would think/hope.  I posted a link to The Horse magazine article written about that study on our Facebook page a couple of years ago, and I think it’s well worth a read.  Read it here.

To bring that home for you, many people will have heard of the tragic, fatal cases of selenium toxicity that occurred a few years ago in a group of polo ponies fed a feed that was made with far too much selenium.  The manufacturer had simply made an error in calculations and had put a decimal in the wrong place, resulting in 10 or 100 times the selenium being added to a batch of feed.

In the racing industry at the moment, the trace-element, cobalt, required for health in small amounts, has been in the news lately. Excessive levels found in urine constitute a positive test, and several cobalt positives have recently been or are currently being investigated by racing authorities.  In some of the cases, injectable products were found to be at fault, but in one case, a powdered feed supplement which contained cobalt levels far in excess of label claims has been implicated.

We have done a cobalt clearance study that you might want to read, and we test, both, our raw ingredients and our finished product for cobalt to ensure that the exact dose listed on the label, not more or less, is what is in BOOST.

Almost everyone in racing will know of someone who has ended up with a caffeine positive as a result of feed contamination, and I believe there was a recent case in European equestrian circles in which a feed, contaminated with poppy seeds, resulted in a positive test and the disqualification of a prominent horse and competitor.

So, how do you know if a product is manufactured safely and meets label claims?

This information isn’t generally on the label, but it can be just as important as the label itself.  To get it, you either have to know the company management personally and have confidence in their diligence and ethic; you have to actually talk to the manufacturer and ask questions; or you can look at their website to find a statement about quality management or evidence of third party certification of their quality management practices.

Tip: Be sure to ask every rep that visits your stable about quality management as they will almost certainly be the most readily available source for this information. That will also be a simple way to separate the wheat from the chaff. Any rep that can’t talk competently about their company’s quality management program probably represents a company that doesn’t have one.

Does the manufacturer have a quality management program?  GMP or ISO certification provides hard evidence of this.

GMP stands for Good Manufacturing Practice, and this is a specific standard required for pharmaceutical producers.  It is, however, voluntary for feed supplement manufacturers.  A generic version of good manufacturing practice, abbreviated with small “gmp”, is a reference to a quality management system that is not name-brand, government specified and inspected, GMP.  It could be the same as GMP or it could be applied to a non-standardised or less complete quality system.

Compliance with a name-brand GMP program ensures that quality is built into the product at the time of manufacture and provides assurance that products are consistently manufactured from quality inputs; in a safe and clean environment; by trained and diligent staff; using carefully defined procedures.  It is a means of giving consumers confidence that products meet the required quality standards, are safe, and are reliable.  A documentation trail that links starting materials, through the various manufacturing processes, to the finished product confirms that dispatched product has been approved through quality control measures.  It also ensures that product can be tracked and recalled if any issues arise.

ISO9001/22000 refer to slightly different quality management standards that do not relate directly to pharmaceutical production but that cover many of the same principles.  They demonstrate a commitment by management to food safety, and they ensure that consideration of potential hazards and critical processes have been considered in the development of the product and production procedures.

If a company has either ISO or GMP certification, you can be sure that the supplements they produce will be safe, secure, and generally meet label claims.

If a manufacturer lacks certification, it doesn’t mean they aren’t doing a fabulous job of quality management.  They might have a written statement about their commitment to quality management or you might have to ask some questions to be sure.  If at least some proportion of finished product undergoes analysis for common contaminants, the concentration of active ingredients, and microbial testing, it will likely be safe.  If no testing is done, and the company doesn’t talk about product quality, safety, and security, I’d worry.

FYI, Pro-Dosa International Ltd. is GMP certified by the Government of New Zealand, Ministry for Primary Industries, Agricultural Compounds and Veterinary Medicines group.  Our quality management system has been in place since 2005, and it was originally developed along GMP, ISO22000, and ISO9001 standards.  The whole team is very proud of our standards, and we would be happy to tell you all about what we do, if you really want to know.

Make sure you read the final installment in this series of blog posts on Reading Feed Labels.  This article discusses the requirements in regards to nutrient composition of horse feeds. Part 4 – Feed.

07 May

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Now that you have supplements sorted out, what about feeds?  Comparing prepared feeds is just about as confusing as comparing supplements, but with less math, you’ll be pleased to know

Somehow, until the mid-2000’s, I managed to go through life blissfully unaware of the complexities of choosing prepared feeds.  I worked in racing stables, and all my clients fed a prepared racing formulation to their racehorses.  Though some added bits and bobs, such as barley, supplements, and oils, they didn’t really ask me that many questions about their basic feeding regimens.  Then, I set up a mobile equine veterinary practice in Al Wathba, Abu Dhabi, UAE.  It was a pretty interesting place where I met a broad range of people, from nearly every background and nationality, with a wide range of horses, in a whole array of different management situations, doing every possible activity.  To add to the rich tapestry of experiences, I’d often find the entire equine and human smorgasbord at a single stable.

This complex set-up, the need for refrigerated feed rooms (Outside temperatures soar to 45-50 degrees Celsius, and inside it would be even hotter.  Nutrients aren’t very stable when they are heated excessively, and moulds etc. do really well.), and the ordering systems for some feeds meant that many of these stables had an entire pallet of feed, for each type of horse, in each activity.  This, of course, cost a fortune and took up way more space than anyone really had in their refrigerated feed rooms.  To top it all off, the forages available (…and there is no grass…ever), are variable in quality throughout the year.  Some had imported hays that were expensive but quite nice for at least part of the year, and some had the highly variable and often not very nutritious local hay.  This meant that feed costs and logistics were of major concern to virtually all of my clients.  They pretty much all asked me about the prepared feeds they were feeding and asked about simplifying the whole ordeal.  (They also asked me about the value of dates and camel’s milk in the equine diet which I looked into, being open minded and all.  I was a bit impressed with camel’s milk in particular and would quite like a dairy camel at home in NZ… I’ll tell you about what I found out sometime…but I digress…)

In order to answer all the questions about feeds I was getting, I took myself off to the camel souk (a market at the camel racetrack where, ironically, most horse products can be found) and had a look at the myriad feed stores.  There were feeds from every company I could think of, from every country in the world.  It would take a lifetime to sort through the entire selection, so I started by looking at the ranges of feeds from each of the brands that my clients were feeding.

I found an enormous number of feeds that looked pretty similar.  I had a hard time telling apart…

  1. the different formulations from the same company as well as
  2. the formulations from different companies, all of whom had similar feeds and ranges as the others.

The bags were different, but what was in them was pretty much the same. They all had very similar ingredients, in very similar, but not quite identical, proportions. I couldn’t really tell them apart, and I’m guessing, without referring to the names on the bag, like Racehorse Mix or Cool Feed, you would struggle to tell the difference too.

Before we begin sorting out how to compare different feeds, you should know some feed basics.

It is important to know something about the volume a horse will eat in a day.  Most horses will eat between 2-3% of their body weight per day in feed, including hay, grass, and concentrate feed.  When horses are working very hard, their feed consumption is generally at the bottom of the range, and for that reason, they can’t normally meet their protein and energy requirements by eating grass or hay.  A concentrated form of those nutrients must be fed.

Since a 500kg horse in hard work will only eat about 10 kg per day, all of the nutrients required must be fed in that volume of feed. It is also important to keep in mind that the larger part of the ration should be provided in the form of roughage as that is what keeps the gut moving properly and the flora healthy.  For this reason, you really can’t just feed a horse 10kg of a concentrated feed and nothing else.

So, before we begin to talk about feed, then, we have to talk a bit about hay and grass.  It is important to know something about the quality of your forage.  You can use average values for the nutrient content for each plant species to get a rough idea of what your hay or grass is contributing to your horses’ daily rations, but an analysis provides more accurate information.  You may be able to ask your feed supplier for an analysis of the hay they sell, or you may send samples of your pasture grass and hay to a laboratory yourself.  There are also packaged hay products available in most parts of the world.  These are labelled with nutritional information, just as concentrate feeds are, but there is one catch you may not be aware of.  Nutritional information can be listed on a “Dry Matter Basis” or on an “As Fed” Basis.  “As Fed” is pretty self-explanatory.  Nutrient content is measured in a kg of the forage in the form that you’d feed it.  When you feed 1 kg of it, you will be feeding the feed, moisture and all. “Dry Matter Basis” means that the nutrients are listed as a percentage of just the dry component of feed that is left when the moisture is removed. When you compare feeds, you will need to calculate the amount of nutrients on an “As Fed Basis”, as when you actually feed your horse, you will be feeding the feed, moisture and all.

For example, if a packaged forage lists their nutrient content on a Dry Matter Basis, but the moisture content is listed 50%, 1kg of that forage contains only 500g of dry matter and 500g water.  If the label says there is 20% protein on a dry matter basis that is the same as saying there is 200g of protein per kg of dry forage.  When you actually feed it to your horse, however, you will be feeding the forage along with an equal amount of moisture (water).  To get 1 kg of dry matter from that feed, therefore, you have to feed 2 kg.  If 1kg of the feed, on a dry matter basis has 200g of protein, when the 1kg of moisture is added back into the calculation, there is only 200g of protein in 2 kg of the feed as fed.  On an as fed basis therefore, the feed is only 10% protein or 100g of protein per kg of feed.  In this example, content “as fed” = 20% protein on a DM Basis x 50% moisture/100%

To convert from dry matter basis to as fed basis, multiply by the percentage moisture and divide by 100.

With dry hays, the moisture content is less than 10%, so the “as fed” content is almost the same as the content on a dry matter basis.  Grass, in contrast, in about 90% water, so the as fed content is very different than the content on a dry matter basis.

Depending on the maturity and conditions at harvest as well as the type of hay, protein content can vary from well under 10% for poor grass hay up to 18-20% or so for nice Lucerne (alfalfa) hay, chaff, or cubes.  The poorer the hay being fed; the greater the importance of the hard feed.

Horses need energy, protein, calcium balanced with phosphorus, vitamins, and minerals including salt.  All horses need these same nutrients, but those working hard, growing, in foal, and lactating, need more of them.  You can find nutrient requirements for your specific horse by looking up NRC or by asking your feed supplier, nutritionist, or veterinarian.  I talk about some of the requirements later in this post.

When nutritionists balance rations, they start by looking at the protein and energy content (and costs) of the feed ingredients available. Then, once they have decided on the main components, they look at vitamins and minerals etc. which can be added and adjusted to achieve optimal balance while considering the contribution of the main components of the feed.  So, just as if we were formulating a new feed or ration, we will start with energy and protein.

The main differences between the varieties of commercial preparations include…

  1. The concentration of nutrients, especially energy and protein, and
  2. The specific form of energy and protein provided.

For example, spelling mixes have lower concentrations of energy and protein than racehorse mixes.

This is generally because most feeds are designed to be fed at a rate of 4-6 kg per day.  A spelling horse will need to eat 5kg of a spelling mix to meet its lower requirements for energy and protein, and a racehorse needs to eat 5kg of a racing mix to meet its needs.  In fact, if the spelling horse ate the racehorse mix, it could just eat less of it to get the required amount of nutrients.

Protein Quantity

For this section, remember that there are 1000 grams (g) in 1 kilogram (kg).  I’m sorry, but I didn’t promise there wouldn’t be ANY math; just less of it.

Protein is often expressed as a percentage on feed bags, and horsemen tend to think of protein requirements in terms of those percentages.  (ie.  Racehorses need 16% protein and spelling horses need 10%.) In fact, protein requirements are in grams per day and not in percentage at all.

100g of protein could be found in 100g of a 100% protein feed; 1kg of a 10% protein feed; or 10 kg of a 1% protein feed.  So, the percentage of protein in a feed is only important when you consider how much of that feed your horse will eat.

Horses in hard work need about 1000-1500 g, particularly if they are also growing.

Spelling horses need about 750 g

Ponies (adult weight 200 kg) in hard work need 350-450 g

The average 2-3 year old racehorse in intense work needs 1000-1500g of quality protein per day.  As I already said, a horse in hard work will eat 2% of their body weight per day.  As a result, a racehorse could be expected to eat only about 5kg of concentrate feed and 5kg of roughage.  If the average protein content of hay is 13%, then about 650g of protein can be derived from hay.  The rest has to come from the concentrate feed.

A concentrate feed of at least 13% protein fed at a rate of 5 kg per day might be enough for the average horse, but some will need more like 850g of protein from their concentrate feed, and since that must be provided in 5kg or less feed, then the feed would have to be 17% protein to meet requirements.  If it was 20% protein, the 850g could be fed in 4kg of feed, and the 650 would be provided in 3kg.  That would allow that horse to eat a bit more hay, which has some benefits for the health of the gut.

Remember, don’t worry about the percentage of protein in the feed being high.  If the percentage of protein in the feed is high, you will simply be able to feed less of it to meet your horse’s protein requirements.

Protein Quality

In order for horses to make proteins (which are the building blocks of muscle cells), 10 essential amino acids must be provided in particular ratios. (Essential amino acids are those that horses cannot synthesise and, therefore, must consume.)  If even one of these essential amino acids is in short supply, protein production will stop. The amino acid, available in the lowest relative amount, is said to be the limiting amino acid.  The excess amino acids (the ones in higher amounts than the limiting amino acids, that now can’t be used for protein and muscle cell synthesis in the horse) become waste products, resulting in increases in heart rate and load on the kidneys.  For this reason, the best protein sources have the best essential amino acid balance, and as many of the amino acids provided as possible can be used for protein synthesis and muscle development in the horse.

Grains, like barley and oats, have some imbalances in the amino acid ratios, and as a result, only about 40% of the protein in an oat or barley-based feed will actually be useable by the horse to make muscle. In comparison, the ratio of essential amino acids in peas, lucerne (alfalfa), lupins, or soy allows for about 80-90% of the protein to be used.

Make sure you consider, therefore, the type of protein provided in a feed.

Energy

  • Horses in hard work need about 26-35 Mcal of DE (Digestible Energy)
  • Spelling horses need about 15-18 Mcal of DE
  • Ponies (200kg mature weight) in hard work need 11-14 Mcal of DE

Energy requirements can often be met by pasture or hay in spelling horses, but for horses in hard work, it is necessary to feed a concentrated feed in addition to forage.  Energy is provided in equine feeds in the form of carbohydrate (grains, corn, etc.) and as fat.  Traditionally, oats and barley were fed as the primary energy source.  They are a cost-effective way to provide energy, and some horses do very well on them.  Some individuals, however, do not.  Horses that suffer from a tendency to tie-up, those that have a tendency to founder, and those that colic are generally better avoiding grains.

Normal carbohydrate digestion starts in the small intestine.  Starch is broken down to complex sugars, and the complex sugars are, in turn, broken down to glucose.  Glucose is absorbed through the small intestine.  Any starch or sugars that aren’t digested and absorbed by the time the digesta leaves the small intestine are dumped into the cecum and large intestine.   That part of the gut, known as the hindgut, is a big fermentation vat. Bacteria in the hindgut work slowly on the digesta, breaking down roughage to molecules called volatile fatty acids that can be used by the horse for energy.

Horses’ guts, however, were never designed to process large amounts of starch, so the enzymes required for its digestion are produced in limited supply.  Grains are very high in starch and sugar and can, therefore, pose problems for digestion.  If large amounts are fed at one time, some starch and sugar pass undigested into the hindgut, making it’s environment more acidic.  The beneficial population of bacteria can’t survive in an acidic environment, and when they die, they release endotoxins.  Those endotoxins are responsible for problems such as colic, laminitis, and diarrhoea.  In addition, undesirable bacteria are able to thrive, further disrupting the cecal pH and worsening the environment for beneficial bacteria and so on.

While the rule of thumb is to limit grain feeding to 2kg at a single meal, some susceptible individuals will not even tolerate that quantity at once.

In comparison, fat is a reasonably safe source of energy.  While horses were not really designed to eat fat either, they do not seem to have problems with it.  Researchers have found that horses tolerate as much as 10% of their diet as fat.  For horses in hard work who may require quite a bit of extra energy in their concentrate feed, or for individuals prone to laminitis etc, look for a feed that has less grain and more fat.  It can take muscles cells 2-4 weeks to adapt to fat as an energy source, so if you are switching a racehorse to a high fat, low soluble carbohydrate diet (low grain), try to do it when they are not racing in a week.  Once metabolism adapts to fat, there is evidence of improved endurance, lower body temperatures, and a reduced incidence of tying-up, laminitis, and colic.

Vitamins, Trace Minerals, and Extras

Pasture and hay may not meet all of the vitamin and trace mineral requirements, even in spelling horses.  These nutrients, in most cases, will need to be supplemented, either on top of a plain feed, or in the form of a prepared feed.   I wrote about vitamin and mineral requirements as well as the need for balance between each of these elements in the first parts of this reading labels series, so I’ll just mention a few things here.

I generally prefer people to provide a prepared feed rather than mixing their own, unless they know a bit about nutrition or have had advice from a nutritionist.  It is easy to get the balance wrong and cause more problems than you fix.  Companies making feeds employ nutritionists and provide reasonably balanced preparations for you.  I would go so far as to say that all feed companies refer to nutrient requirement standards when formulating their products.  In reviewing the huge range of feeds available in the UAE and New Zealand, all I looked at appeared to meet the basic, daily requirements for fat soluble vitamins and trace elements when fed according to package directions.  Some provided those nutrients in more bioavailable forms than others (see part 2 of our reading labels post), and I would certainly choose the feeds that include more bioavailable forms of nutrients, but most would be pretty acceptable.

Calcium and Phosphorus must be provided in the diet of horses in a 1.5-2:1 ratio. Commercially prepared feeds will all be pretty well balanced in this department, so you won’t have to worry about this much.   In general, grains are high in phosphorus, while lucerne (alfalfa) is high in calcium. Be careful to get this right if you are mixing your own feeds or adding additional grains to prepared feeds.

I talked about most of the trace minerals in previous sections of this marathon post about reading labels, but I didn’t say much about selenium.  NRC says that horses in hard work require 1 mg per day, and most nutritionists would probably agree that 3mg is a better dosage to aim for in the diet.  The interesting thing about the requirements, is that horses really don’t have a requirement for elemental selenium (just plain, selenium).  Their requirement is actually for the selenium containing amino acids, seleno-methionine and seleno-cysteine.  This is important.

Selenium is described as having a “narrow therapeutic range”.  What that means is that the amount that is toxic for horses is not that much more than the amount they require for normal health.  This is also important to know.

Selenium can be provided in several forms.  It is provided in feeds as sodium selenite, sodium selenate, selenium yeast, seleno-methionine, and seleno-cysteine.   These forms have some fundamental differences.  They are not all absorbed, used, and excreted by animals to the same extent.  Selenium yeast is a form of selenium that is created when yeasts are grown in an environment with lots of selenium.  The yeasts incorporate the selenium into their own amino acids, so the selenium in selenium yeast is actually provided as seleno-methionine and seleno-cysteine for the most part.  So, you can consider the last three forms as essentially equal.  They are organic molecules that the body is good at absorbing and using.

The sodium selenite/selenate molecules, on the other hand, are inorganic salts that are unpredictably absorbed and used by animals.  Studies done about 20 years ago in dairy cows demonstrated that cows supplemented with the same, standard amounts of those forms of selenium in the same diets, ended up with very different levels of selenium in their blood.  Some were in the normal range, and some were deficient.  When the level of supplementation was increased to correct the deficiencies seen in part of the herd, others started to show signs of toxicity.  The conclusion was that the ability to absorb and use sodium selenate and sodium selenite was very individual and quite unpredictable.  It was recommended to measure blood selenium in each animal before deciding on the appropriate dietary amount of selenium to feed.  When the herd was placed on organic forms of selenium instead, the absorption and use was much more uniform across all individuals in the population.  I haven’t found a link to this study to post, but Alltech, a supplier of vitamin and mineral supplements, used this study in their promotional info in Canada probably 20 years ago, so you might have a look at their website for more details about selenium yeasts.  Dr Pagan from KER did a study that demonstrated much higher bioavailability of yeast-based selenium compared to inorganic forms, (Link to study) and many other researchers have demonstrated similar results with all the organic forms.

The other thing that is worthwhile noting is that inorganic selenium is not excreted very easily.  If you end up with a low grade toxicity, it can take months for the levels to drop back down to normal.  If you feed a feed that has selenium in one of those forms, it’s fine, but make sure you have your veterinarian check blood selenium levels from time to time and follow their recommendations for supplementation.  Please don’t sprinkle inorganic selenium on your feeds willy-nilly!  More is NOT better in this case!

The moral of that story is… when you are comparing different feeds, try to choose one that includes selenium in an organic form.  The chances of having normal blood selenium levels will be improved.

Finally, the end is near!

To summarize, evaluate your forage and determine how much additional protein and energy will be required from the concentrate portion of the ration.  Then, you can probably just pick a company that you like to deal with, as most have similar ranges.   Pick a feed from their range that has nutrients in a high enough concentration that your horses in intense work can meet their protein and energy requirements by eating no more than 5kg of hard feed so they can eat 5 kg of hay (or other forage) to meet fibre requirements and to maintain healthy gut motility etc.  Try to pick a feed that has a good quality protein, so pick one with a bit more soy, peas, lupins, or alfalfa, rather than just oats and barley.  If you want to avoid problems related to high carbohydrate diets, then look for higher fat diets with little or no oats and barley.   Finally, have a look at the trace minerals provided in the daily ration.  Pick a feed that has bioavailable forms of trace minerals in proper balance with each other.

Once you have selected a good quality, safe, and healthy feed, then you can probably feed it to most of the horses at your stable.  Spelling horses and smaller horses will need to eat less of it with more hay or grass.  Racehorses or broodmares etc will need to eat more of it.

Best of luck with choosing feeds.  Remember, we are happy to help you read feed and supplement labels, and we are happy to do feed comparisons for you contact us now.

If you haven’t read the proceeding articles on Reading Feed Labels follow the link to start at the beginning Reading Labels Part 1 – Supplements – Comparing Apples to Apples?